egg antibodies
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Hassl codicillo quarto regularum vittellorum gallinaceorum causa
Experimental studies on the demonstration of egg-yolk immunoglobulin Y against Toxoplasma gondii. |
Hens were immunized with aqueous extracts of Toxoplasma gondii trophozoites, eggs were collected, and egg yolk IgY-antibodies were isolated by PEG precipitation. Gel filtration was done for separation of antibodies from residues. In an Ouchterlony-test Toxoplasma-antigen was precipitated with IgY-antibodies an one hand and conventionally raised rabbit IgG-antibodies an the other, and the precipitation lines were compared. | The production of specific IgY-antibodies offers important advantages particularly with regard to modern animal protection regulations and work facilitation. Nevertheless, a considerable disadvantage is that different antigen constituants are precipitated by IgY- and IgG-antibodies. Thus the use of egg yolk IgY as an alternative source of specific antibodies for serology of toxoplasmosis needs further investigations. |
Hassl codicillo quarto regularum vittellorum gallinaceorum causa
Comparative studies on the purity and specificity of yolk immunoglobulin Y isolated from eggs laid by hens immunized with Toxoplasma gondii antigen. |
Eggs from immunized hens were used as a source of yolk antibodies directed against soluble proteins of Toxoplasma gondii. The yolk immunoglobulins (IgY) were extracted and purified by the use of three different methods. The antibody yield and the purity of each batch were determined by protein measurements, gel filtration and isoelectric focusing. A sequence of two precipitation steps, i.e. a precipitation by polyethylene glycol followed by an alcohol treatment, was shown to be the most effective purification method. | The specificity of the yolk antibodies was evaluated by means of an indirect hemagglutination assay, an immunodiffusion test and an immunoelectrophoresis. It was compared with the specificity of IgG antibodies obtained from sera hyperimmunized rabbits. The precipitation patterns of IgY and IgG antibodies were non-identical. This results indicates differences between the specificities of egg yolk IgY antibodies and rabbit IgG serum antibodies, although both animal species had been immunized with identical antigen preparations. |
Hassl codicillo quarto regularum vittellorum gallinaceorum causa
Purification of egg yolk immunoglobulins. A two-step procedure using Hydrophobic interaction chromatography and gel filtration. |
 A two-step chromatographic procedure was developed for the isolation and purification of hen IgY antibodies from egg yolk. The antibodies were completely separated from vitellin and lipids by hydrophobic interaction chromatography followed by gel filtration. | Almost no residual yolk proteins, no immunoglobulin aggregates, and no antibody fragments could be detected in the final extract. Moreover, the method described, guarantees the recovery of antibodies of undiminished activity. Although the final yield is somewhat lower than that obtained by an isolation method consisting of two precipitation steps with polyethylene glycol and alcohol respectively, the procedure described is particularly recommended when highly purified antibody preparations are needed. |
Hassl codicillo quarto regularum vittellorum gallinaceorum causa
Snake egg immunoglobulins: biochemical characteristics and adjusted isolation procedure. |
Transmission of specific immunoglobulins from mothers to their offspring via the egg is a common phenomenon in egglaying vertebrates but the occurrence of this phenomenon in reptiles, especially in colubrid snakes, has not been proven until recently. Thus, the essential biochemical characteristics of antibodies deposited in eggs of Elaphe guttata (Colubridae, Serpentes) were studied after isolation of the antibody by precipitation and purification by | affinity chromatography and gel filtration, with verification by isoelectric focusing and immunoprecipitation. The immunoglobulin deposited in the eggs of colubrid snakes is a singular, non-truncated IgY antibody in a concentration roughly equal to that in the snake's serum. An efficient method to isolate antibody from snake eggs was developed, based on the PEG precipitation technique of avian yolk immunoglobulins; an unsophisticated protocol for the isolation procedure appropriate for reptile eggs is provided. |
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